![]() ![]() Milk and blood samples were obtained from 96 BLV-positive dams and extracted DNAs. Positivity rates for bovine leukemia virus (BLV) provirus and estimation of proviral load (PVL) in milk from susceptible, resistant, and neutral dams. This is the first report confirming that BoLA-DRB3 polymorphism affects the PVL and infectivity of milk from BLV-infected dams.īoLA-DRB3 allele bovine leukemia virus (BLV) dam infectivity milk proviral load resistant susceptible vertical transmission visualization. The visualization of infectivity of milk cells using a luminescence syncytium induction assay showed that the infectious risk of milk from BLV-infected dams was markedly high for susceptible dams compared to resistant ones. This result was confirmed in a three-year follow-up study in which milk from susceptible dams showed a higher BLV provirus detection rate over a longer period than that from resistant dams. The detection rate of BLV provirus and PVL were significantly higher in milk from susceptible dams than in that from resistant dams. This study examined milk from 259 BLV-infected dams, including susceptible dams carrying at least one BoLA-DRB3*012:01 or *015:01 allele with high PVL, resistant dams carrying at least one BoLA-DRB3*002:01, *009:02, or *014:01:01 allele with low PVL, and neutral dams carrying other alleles. However, the effect of BoLA-DRB3 polymorphism on the infectivity and PVL of milk from BLV-infected dams remains unknown. Bovine leukocyte antigen (BoLA)- DRB3 is a polymorphic gene associated with BLV infectivity and proviral load (PVL). 10 Department of Food and Nutrition, Jumonji University, 2-1-28 Sugasawa, Niiza, Saitama 352-8510, Japan.īovine leukemia virus (BLV), which causes enzootic bovine leukosis, is transmitted to calves through the milk of BLV-infected dams.9 Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, 143 Shimokasuya, Kanagasa, Isehara 259-1193, Japan.8 GenoDive Pharma Inc., 4-14-1 Naka-cho, Kanagawa, Atsugi 243-0018, Japan.7 Laboratory of Farm Animal Internal Medicine, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.6 Laboratory of Animal Health II, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Kanagawa, Sagamihara 252-5201, Japan.5 Photonics Control Technology Team, RIKEN Center for Advanced Photonics, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. ![]()
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